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. 2014 Feb 27;4:4217. doi: 10.1038/srep04217

Figure 3. CRISP3 is enriched 15-fold following CM cellulose exchange.

Figure 3

(A) Silver-staining at various purification stages. Lane 1: starting material (250 μl conditioned media), lane 2: CM cellulose flow through, lanes 3–10: CM cellulose exchange gradient elutions (CRISP3 elutes in lanes 7–8, representing 110 and 240 mM NaCl respectively). (B) CRISP3 gel filtration to over 60% purity. (C) Mouse CRISP3 purified by the same method (two bands, 26 and 33 kDa). The 48 kDa contaminant is alpha-enolase. (D) Gel filtration absorbance trace with human CRISP3 peak indicated by the arrow. All gels were run under the same conditions.