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. 2014 Feb 27;4:4217. doi: 10.1038/srep04217

Figure 5. Recombinant mouse CRISP3 is glycosylated at N99 and N113.

Figure 5

Western blotting with His tag antibody showed glycosylated human and mouse CRISP3-His to be 29 and 33 kDa respectively (A). (B) Peptide pre-absorption of mouse CRISP3 antibody. (C) Mouse CRISP3 N99Q and N113Q mutant proteins were smaller than wild type (WT) mouse CRISP3 (PNGase F untreated samples); N156Q was unaffected. Two mutants unable to undergo glycosylation (N99Q + N113Q and N99Q + N113Q + N156Q) were of relatively low abundance. ‘NT' represents non-transfected HEK cell media. Full length gels are available under supplementary data; gels were run under the same conditions.