Table 1. CRISP3 purification from HEK 293 conditioned media.

Step	Volume (ml)	Protein (mg)*	hCRISP3 (μg)†	Purification (fold)	Recovery (%)
1. Media	500	40	720	1	100
2. CM cellulose	10	2.2	540	15	75
3. S75	10	0.75	500	37	70

*Determined by Bradford assay.

^†Determined by western blot comparison with commercial HIS-tagged CRISP3 standard and densitometry of silver-stained SDS PAGE (steps 1 and 2–3 respectively).