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. 2014 Feb 27;4:4220. doi: 10.1038/srep04220

Figure 2. LPS increases SIRT1 expression by IFN-β mediated signaling pathway in BMDMs.

Figure 2

(A) Dose dependant effect of IFN-β on SIRT1 protein expression. BMDMs were incubated with the indicated doses of IFN-β for 24 h. A western blot was performed for SIRT1 with β-actin as a loading control. (B) The effect of IFN-β neutralizing antibody on LPS-induced SIRT1 expression. BMDMs were treated with LPS in the presence or absence of IFN-β neutralizing antibody (1 μg/mL) for 24 h. (C) The effect of various inhibitors for signal transduction on IFN-β-induced SIRT1 expression. BMDMs were pretreated with vehicle (DMSO), 10 μM PD098059 (PD), 10 μM SB203580 (SB), 10 μM SP600125 (SP), 10 μM PDTC, 1 μM wortmannin (WT), 5 μM LY294002 (LY), 5 μM JAK1 inhibitor (JAK1), and 10 μM JAK3 inhibitor (JAK3) for 1 h and further treated with 100 U/ml IFN-β for 24 h. Full-length blots are presented in Supplementary Figure S3–S5. The Western blot shown is a representative of three independent experiments.