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. 2014 Feb 27;4:4219. doi: 10.1038/srep04219

Figure 4. Wild type Rac1 interaction with importin-α2 during the DNA damage response.

Figure 4

(A)–(B) Intensity image of a NIH3T3 nucleus transiently transfected with Rac1-CyPet importin-α-GFP respectively, before (left) and immediately after (right) induction of DNA damage. (C) Molecular brightness of Rac1-CyPet before (left) and after (right) DNA damage pseudo-coloured according to the coloured cursors superimposed over the brightness versus intensity distributions: monomers coloured green and dimers coloured red. Dimers are exported from the nucleus upon induction of DNA damage. (D) Molecular brightness of importin-α-GFP before (left) and after (right) DNA damage pseudo-coloured according to the coloured cursors superimposed over the brightness versus intensity distributions: monomers coloured green and dimers coloured red. Dimeric population along the nuclear envelope is disassembled upon induction of DNA damage. (E) Cross variance analysis of Rac1-CyPet with importin-α-GFP in each pixel of the image, before (left) and after (right) DNA damage. A nuclear association of Rac1-CyPet with importin-α-GFP is induced in addition to a cytoplasmic association, upon induction of DNA damage.