Figure 6. DYF13 does not influence IFT particle movement.
(A) Kymographs of KAP-GFP in control and dyf13 mutant C. reinhardtii cells. These kymographs were assembled from Videos 9 and 10. Bars: 5 s (horizontal), 5 μm (vertical). (B) The mean IFT speeds of KAP-GFP in control and dyf13 mutant. (C) The mean frequencies of KAP-GFP in control and dyf13 mutant in the same range of flagella length. The mean speeds and frequencies of KAP-GFP in control (light blue) and dyf13 mutant (red) were almost same in the same range of flagella length (6–8 μm). (D) Kymographs of IFT27-GFP in control and dyf13 mutant C. reinhardtii flagella. These kymographs were assembled from Videos 11 and 12. Bars: 5 s (horizontal), 5 μm (vertical). (E) The mean IFT speeds of IFT27-GFP in control and dyf13 mutant in the same range of flagella length. (F) The mean frequencies of KAP-GFP in control and dyf13 mutant in the same range of flagella length. The mean speeds and frequencies of IFT27-GFP in control (light blue) and dyf13 mutant (red) flagella were almost same in the same range of flagella length in both anterograde and retrograde. Error bars represent standard deviations.