Video 4. High-speed DIC videomicroscopy of KV cilia from ttc26 knockdown embryos.
A dorsal view of the KV from a live ttc26 knockdown embryo at 8–10-somite stage. DIC imaging was performed on an inverted microscope with a high-speed camera. Images were collected at 1000 fps for a total duration of 1 s. Playback is set at 20 fps (1/50 speed). The cilia show a circular motion but with a slower mean frequency of 17.7 ± 3.3 Hz and sometimes showed unusual beating motion.