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. 2014 Mar;184(3):631–643. doi: 10.1016/j.ajpath.2013.11.019

Figure 3.

Figure 3

Lacrimal gland evaluation in young (8 weeks), middle-aged (6 to 9 months), and old (24 months) C57BL/6 mice. A and B: Tear volume (A; means ± SD) and tear EGF concentrations (B) were measured by enzyme linked immunosorbent assay. Tear washings from both right and left eyes from one mouse per age and sex were collected and pooled into a single tube, yielding a final sample of 12 individual samples per group and age divided into three independent experiments with four samples per experiment). Bar graphs show the means ± SD. C and D: Representative images of lacrimal gland frozen sections immunostained for CD4 (in red, C) and CD8 (in red, D). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 for within strain comparison; ††P < 0.01 for sex comparison. Original magnification: ×40 (C and D). EGF, epidermal growth factor; 8W, 8 weeks; 6-9M, 6 to 9 months; 24M, 24 months. Dashed lines indicate female-to-female comparison; solid lines indicate male-to-male comparisons.