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. 2014 Mar;184(3):631–643. doi: 10.1016/j.ajpath.2013.11.019

Figure 4.

Figure 4

Flow cytometric analysis of LG in young (8 weeks), middle-aged (6 to 9 months), and old (24 months) C57BL/6 mice. Right and left extraorbital LGs from one mouse per group and sex were excised and pooled into a single tube, yielding a final sample of 12 individual LG samples per group and age divided into three independent experiments with four samples per experiment). Bar graphs show means ± SD. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 for within strain comparison; †P < 0.05 for sex comparison. LG, lacrimal gland; MHC, major histocompatibility complex; TCR, T-cell receptor; 8W, 8 weeks; 6-9M, 6 to 9 months; 24M, 24 months. Dashed lines indicate female-to-female comparison; solid lines indicate male-to-male comparisons.