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. 2014 Mar;184(3):686–696. doi: 10.1016/j.ajpath.2013.11.032

Figure 2.

Figure 2

Reduced ischemia injury and improved arterial growth in thigh muscles of kPhd2KO mice. A and B: Detection of apoptotic myocytes (brown) by anti–activated caspase 3 IHC staining. Adductor muscle cross-sections used in this assay were prepared 3 days after FAL. Images in the WT group were taken from necrotic areas. In kPhd2KO mice, necrotic areas were not found in adductor muscle sections. C and D: Anti-CD31 and anti–activated caspase 3 double IF staining of adductor muscles 3 days after FAL. Images in WT group were taken and quantified from areas adjacent to severely necrotic tissues. Images in kPhd2KO sections were taken from random areas. E and F: Anti-CD31 and anti-Ki67 IF staining of adductor muscle sections 3 days after FAL. Double-positive cells are indicated by arrows. Images were taken from similar areas as described for C and D. G: H&E-stained sections of semimembranosus muscles on day 28, with arterial lumens indicated by arrows. H: Measurements of arterial lumen diameters. n = 7 (A, B, G, and H); n = 3 (C–F). ∗P < 0.05, ∗∗P < 0.01 (B, D, F, and H) by Student's t-tests. Scale bars: 100 μm (A and B); 20 μm (C–F); 10 μm (G).