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. 2014 Mar;184(3):686–696. doi: 10.1016/j.ajpath.2013.11.032

Figure 4.

Figure 4

Differential calf muscle regeneration and toe protection among WT, kPhd2KO, kDKO, and kTKO mice. All sections were from gastrocnemius muscles 28 days after FAL. A and B: Anti-CD31 staining and quantification of CD31+ capillaries. C and D: H&E-stained sections and quantification of areas occupied by healthy (non-necrotic) muscle bundles. Necrotic muscle tissues were excluded from quantification. Examples of non-necrotic muscle bundles are demarcated by dotted lines in C. E and F: Gomori trichrome-stained gastrocnemius muscle sections and quantification of fibrotic area (blue). G and H: Anti-CD45–stained sections and number of CD45+ cells (brown dots) per high power field. I: Paw and foot necrosis 28 days after FAL; blackened nails or nail loss are indicated by arrows. J: Necrosis scores from day 0 to day 28. n = 7 to 10 (A–J). ∗P < 0.05 (F and H), ∗∗P < 0.01 (D) by Student's t-tests; ††P < 0.01 (J) by ANOVA). Scale bars: 15 μm (A); 50 μm (C and G); 100 μm (E).