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. 2014 Mar;184(3):827–841. doi: 10.1016/j.ajpath.2013.11.016

Figure 3.

Figure 3

Increased erythropoiesis by activation of HIF signaling and up-regulation of HIF target genes in 6-week-old klotho−/− mice. Real-time quantitative PCR of renal Epo mRNA (WT, n = 5; Klotho−/−, n = 5) (A), BM Epo mRNA (WT, n = 14; Klotho−/−, n = 6) (B), and liver Epo mRNA (WT, n = 9; Klotho−/−, n = 7) (C). D: Serum Epo levels (WT, n = 14; Klotho−/−, n = 14). E: Real-time quantitative PCR of HIF-2α in kidney (WT, n = 6; Klotho−/−, n = 5), liver (WT, n = 5; Klotho−/−, n = 4), and BM (WT, n = 6; Klotho−/−, n = 4). F: Liver transferrin (WT, n = 5; Klotho−/−, n = 6). G: Liver transferrin receptor (WT, n = 6; Klotho−/−, n = 6). H: Liver glucose transporter type 1 (glut-1) (WT, n = 6; Klotho−/−, n = 6). I: BM transferrin (WT, n = 6; Klotho−/−, n = 4). J: BM glut-1 (WT, n = 6; Klotho−/−, n = 4). K: BM phosphoglycerate kinase 1 (pgk-1) (WT, n = 6; Klotho−/−, n = 4). Data represent the means ± SEM, normalized to HPRT and plotted as fold change over WT (where WT = 1). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001.