Figure 8.

Klotho deficiency affected fetal liver (FL) hematopoietic milieu. Flow cytometry analysis of E15.5 FL cells. Immature erythroid cells (Pro-E; Ter119^medCD71^high) (WT, n = 9; Klotho^−/−, n = 10) (A) and Ery-C fraction (Ter119^high CD71^low FSC^low) (WT, n = 8; Klotho^−/−, n = 6) (B) in Klotho^−/− FLs. C: Colony-forming unit assay of burst-forming unit–erythroid (BFU-E) colonies produced by Klotho^−/− FL cells (WT, n = 7; Klotho^−/−, n = 9). Cells from each mouse were plated in triplicate, and the number of colonies in each plate was counted. Frequency of Lin⁻ Sca-1⁺ c-Kit⁺ (LSK) (WT, n = 10; Klotho^−/−, n = 8) (D) and c-Kit⁺Thy1⁺Lin⁻Sca-1⁺ (KTLS) (WT, n = 7; Klotho^−/−, n = 8) (E) HSC populations in Klotho^−/− FLs. F: Colony-forming unit assay of primitive granulocyte-erythroid-megakaryocyte-macrophage (GEMM) colonies in Klotho^−/− FL cells (WT, n = 7; Klotho^−/−, n = 9). Cells from each mouse were plated in triplicate, and the number of colonies in each plate was counted. Data represent the means ± SEM. ^∗P < 0.05, ^∗∗∗P < 0.001.