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CD169+ infiltrated macrophages are mostly M1 skewed in the spinal cord gray matter of TTBK1 and TTBK1/JNPL3 mice. A: Immunofluorescence of CD169 (peripheral macrophage marker, green) and NOS2 (M1 marker, red) in the L4-L5 spinal cord. Original magnifications: ×10; ×40 (insets). Scale bar = 50 μm. B: Quantification of NOS2+CD169+ cells in gray (white bars) and white (black bars) matter. ∗∗∗P < 0.001, as determined by Student's t-test (N = 4 per group).
