Figure 6.

Reduction of axonal length by the expression of TTBK1 in primary motor neurons. Primary cultured motor neurons were transfected with DNA plasmids encoding GFP and pcDNA3.1 (mock, A) or TTBK1 (B) at 5 days div and fixed at 8 days div. The axonal lengths of transfected cells (as determined by GFP expression) were determined by NeuronJ plug-in and subjected to statistical analysis (C). ^∗∗∗P < 0.001, as determined by Student's t-test (N = 100 per group). Scale bar = 100 μm.