Figure 7.

Microglia-induced neurite extension of primary motor neurons is attenuated by LPS. Mouse primary motor neurons were harvested from embryonic spinal cord and div for 7 days. A: No MCM control. MCM was collected from primary cultured mouse microglia after stimulation with PBS (B), LPS (C), or IL-4 (D), and applied to motor neurons. Cells were fixed 24 hours after the incubation for analysis of neurite density per neuron. E: Quantification. ^∗P < 0.05, as determined by one-way analysis of variance and Tukey post hoc test (N = 50 per group). Scale bar = 10 μm.