Figure 8.

Silencing of endogenous TTBK1 reduces M1-skewed microglia-induced neuronal cell death. A: The silencing effect of lentiviral vectors expressing shRNA targeting different mRNA sequences of murine TTBK1 was quantified by immunofluorescence and presented as percentage TTBK1 intensity versus control groups. B: Primary cultured mouse motor neurons were transduced with siTTBK1 vectors co-expressing GFP at 5 days div, then treated with MCM from PBS- or LPS-treated microglia (PBS or LPS MCM) at 8 days div, and fixed at 9 days div. The number of apoptotic bodies out of siTTBK1 or control viral vector transduced (GFP⁺) cells was counted and presented as percentage cell death over background. ^∗P < 0.05, ^∗∗∗P < 0.001 versus control (A) or LPS MCM (B), as determined by one-way analysis of variance and Tukey post hoc test.