Micro-CT analysis of femur in 4-week-old (A–C), 8-week-old (F–H), and 16-week-old (I–K) D2J mutant and D2J/Gpnmb+ wild-type mice for BV/TV (A, F, and I), Tb.N (B, G, and J), and Tb.Sp (C, H, and K). D and E: Representative three-dimensional micro-CT–reconstructed image of the sagittal (D) and axial (E) planes of the femoral metaphysis, with the corresponding three-dimensional trabecular plate without cortical bone in 8-week-old mice. Data are expressed as means ± SEM. n = 4 (A–C), n = 5 (I–K), and n ≥ 8 (F–H) mice per group. ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗∗P < 0.0001.