Skip to main content
. Author manuscript; available in PMC: 2014 Feb 26.
Published in final edited form as: Mol Cell. 2013 Nov 7;52(3):314–324. doi: 10.1016/j.molcel.2013.10.009

Figure 1. In Vitro Acetylation of K7 Repeats by p300.

Figure 1

(A) Scheme of the GST-CTD wild-type construct and a series of GST-CTD deletion mutants (upper panel) and the in vitro acetylation of these constructs with the purified acetyltransferase domain of p300 and [14C] acetyl-CoA (lower panel). Asterisk marks autoacetylation of p300.

(B) In vitro acetylation of the murine GST-CTD with p300 or PCAF. Reactions were analyzed by autoradiography. p300, but not PCAF, acetylates the GST-CTD construct in a dose-dependent manner. Asterisk marks the autoacetylation band of p300.

See also Figure S1.