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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Cancer Res. 2013 Jul 18;73(17):5569–5579. doi: 10.1158/0008-5472.CAN-13-0677

Figure 2. MUC1 expression by recipient cells in a patient following allogeneic transplantation.

Figure 2

CD34+/lineage− cells were isolated from the bone marrow of a female patient with AML after a sex mismatched allogeneic bone marrow transplant. A. The CD34+ cell population was incubated with a control IgG (left) and anti-MUC1 (right), and analyzed by flow cytometry. B. CD34+ cells were analyzed for MUC1 and GAPDH mRNA levels by RT-PCR. RNA from MUC1+ MCF-7 breast cancer cells was used a positive control. C. CD34+ cells were analyzed for MUC1 expression by immunohistochemical staining (left) and sex chromosomes by FISH (right) using the BioView System. Representative female recipient cells (XX; red signals) and male donor cells (XY; green signals) are highlighted with arrows.