Fig. 3.

Effects of curcumin and Bay 61-3606 on cPLA₂α and 5-LO translocation and MAPKs activation. IgE-sensitized BMMCs were pre-incubated for 1 h with the indicated concentrations of curcumin or Bay 61-3606 and then stimulated with DNP-HSA for 15 min. Cytosolic and nuclear fractions were immunoblotted with antibodies for phospho-cPLA₂α (Ser505) and 5-LO (A), and cell lysates were immunoblotted for the total and phosphorylated forms of ERK1/2, JNK and p38 (B). Immunoblots of β-actin and lamin B were used as controls for cytosol and nuclear fractions, respectively.