Fig. 4.

Effect of Curcumin on COX-2 dependent PGD₂ generation and on Akt-NF-κB activation. IgE-sensitized BMMCs were pre-incubated with the indicated concentration of curcumin or Bay61-3606 for 1 h and then stimulated with DNP-HSA for 7 h. PGD₂ released into the supernatant was quantified using an enzyme immunoassay kit and the cells were used for immunoblotting of COX-2 protein (A). IgE-sensitized BMMCs were pre-incubated with curcumin or Bay 61-3606 for 1 h and stimulated with DNP-HSA for 15 min. Then cells were taken for the immunoblot detection of Akt, IKK, IκBα cytosolic NF-κB (p65), nuclear NF-κB (p65), β-actin and lamin B. Nuclear extracts were used for the NF-κB (p65) immunoblot. Results are presented as mean ± S.D. of three independent experiments. ^**p<0.01 versus the IgE/Ag-treated group.