Fig. 5.

Effect of curcumin on the Syk pathway. IgE-sensitized BMMCs were preincubated with curcumin or Bay 61-3606 for 1 h, and then stimulated with DNP-HSA for 5 min. Cell lysates were subjected to immunoprecipitation and immunoblot analysis for the phosphorylated forms of Syk, LAT and PLCγ1. Bay 61-3606 was used as a positive control with respect to the suppression of the Syk-mediated pathway. The relative ratios of p-Syk/Syk, p-LAT/LAT and p-PLCγ1/PLCγ1 protein levels were determined by measuring immunoblot band intensities by scanning densitometry (^**p<0.01 and ^***p<0.001). The results shown are representative of three independent experiments.