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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Dev Biol. 2013 Dec 24;387(1):73–92. doi: 10.1016/j.ydbio.2013.12.021

Figure 3.

Figure 3

Loss of Kif11 causes monoaster spindle formation in radial glial cells during division. Kif11 siblings and wild type embryos treated with either STLC or DMSO control from 5–30hpf were labeled for radial glia (Gfap), microtubules (α-Tubulin) (microtubules), and histones (Hoechst stain) to identify mitotic spindle phenotypes. (A) Kif11 wild type siblings and (I) vehicle control (DMSO) treated embryos exhibit normal spindles in M-phase (enlarged in B–D and J–L, respectively) while (E) kif11−/− mutants and (M) STLC treated embryos display monoastral spindles characteristic of mitotic arrest (enlarged in F–H and N–P, respectively). White dots designate presumptive locations of centrosomes. For greater clarity Hoechst is displayed as a greyscale image in the enlargements (D, H, L, P).