Kif11 function is most required during two distinct periods for proper cell
division. (A) Schematic illustrating time of exposure to S-trityl-L-cysteine
(STLC) or vehicle control (dimethyl sulfoxide) and duration of treatment between
groups. (B–G) Lateral views of post-treatment embryos labeled for radial
glia (Gfap), Ph3 and AT. (H) In all test groups, there were significantly more
Gfap+/Ph3+ and Gfap−/Ph3+ cells in STLC treated
embryos (E–G) compared with their controls (B–D) at
5–30hpf, 10–30hpf, 15–30hpf, 20–30hpf, and
25–30hpf. Error bars delineate the standard error of the mean. Asterisks
indicate a statistical significance (t-test, two tailed, assuming equal
variances, p<0.05).