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. Author manuscript; available in PMC: 2015 Mar 1.
Published in final edited form as: Dev Biol. 2013 Dec 24;387(1):73–92. doi: 10.1016/j.ydbio.2013.12.021

Figure 8.

Figure 8

Kif11 is required for motorneurons and CoSA interneurons. Wild type/vehicle control (A,C,E,G) and kif11−/−/STLC treated embryos (B,D,F,H) were labeled for anti-GABA (A,B,E,F) to label distinct DoLA, CoSA, VeLD, and KA interneurons (denoted D,C,V, and K, respectively), as well as anti-ISL (C,D,G,H) to label Rohon Beard sensory neurons and motor neurons (denoted R and M, respectively). Decreases in GABA+ cell populations as well as axon pathfinding errors were found in Kif11 deficient embryos as compared with controls (asterisks in B and F label cell body, arrowheads mark axon trajectory). (I) Quantification of neuronal populations identified decreases specifically in CoSA and MN populations within Kif11 deficient embryos (asterisks, p<0.05) as compared with controls. (J) Time course analysis of GABA+ cell types at 24hpf, 30hpf and 36hpf shows a consistent number of GABA+ cells both lost and retained over time in kif11 mutants and STLC treated embryos.