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. Author manuscript; available in PMC: 2014 Feb 27.
Published in final edited form as: Biochem J. 2008 Dec 1;416(2):161–177. doi: 10.1042/BJ20081240

Figure 7. Formation of hetero-oligomers between different CTRPs.

Figure 7

(A–D) FLAG- and HA-tagged adiponectin and CTRPs were co-expressed in different combinations and the supernatants were subjected to co-immunoprecipitations using an anti-FLAG affinity gel and immunoblotted with an anti-HA antibody (top panel). The middle and bottom panels indicate the presence of FLAG- and HA-tagged input proteins in the supernatants. The molecular mass in kDa is indicated on the left-hand side of each gel. IP, immunoprecipitation; IB, immunoblot. (E) Summary of 72 co-immunoprecipitation results (from A–D; Supplementary Figure S3 at http://www.BiochemJ.org/bj/416/bj4160161add.htm). Grey boxes represent homo-oligomerization of adiponectin and each of the CTRPs. Black boxes represent hetero-oligomerization between secreted adiponectin/CTRP2, adiponectin/CTRP9, CTRP1/CTRP6 and CTRP2/CTRP7.