FIG. 6.

GSNO treatment protects H9c2 cells against hypoxic injuries via protein SNO. (A, B) Rat cardiomyocyte H9c2 cells were exposed to normoxia (N) or hypoxia (H) for 4 h in the absence or presence of GSNO (10 μM). (A) Cell morphology was assessed by the phase contrast microscopy. (B) Aliquots of conditioned medium were subjected to LDH activity assay. (C, D) Hypoxic H9c2 cells supplemented with GSNO (10 μM) for 3 h were exposed to HgCl₂ (0.1 or 0.2 μM) for additional 1 h. Normoxic H9c2 cells with the same exposure to GSNO and HgCl₂ were included for comparison. At the end of 4 h of treatment, morphological changes (C) and level of LDH released to conditioned medium (D) of H9c2 cells were analyzed. Data shown in (B) and (D) are presented as mean±SD [n=6 in (B) and n=3 in (D); *p<0.05, **p<0.001]. HgCl₂, mercury dichloride; LDH, lactate dehydrogenase; SD, standard deviation.