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. 2013 Dec 16;6(1):99–119. doi: 10.1002/emmm.201302909

Figure 7.

Figure 7

Pin1 and Fbxw7α modulate N1-ICD stem cell activity in vitro.

A  Fbxw7α overexpression reduces breast CSCs self-renewal. Left panel: %M2FE of MDA-MB-231 cells overexpressing the indicated vectors. Black and grey bars indicate DMSO or GSI treated mammospheres, respectively. Means, standard deviations and P-values (t-test, n = 3), are indicated. Middle panel: Representative microphotographs of M2 are shown, 200 μm scale bar is indicated. Right panel: Western Blot of cell lysates from M2. White and black arrows indicate over-expressed and endogenous Pin1, respectively.

B  Fbxw7α genetic ablation increases breast CSCs' self-renewal in a Notch-dependent manner. Left panel: Histogram showing %M2FE of MDA-MB-231 cells with indicated siRNA and treated with vehicle (black bars) or GSI (grey bars). Means, standard deviations and P-values (t-test, n = 3), are indicated. Right panel: Western Blot of cell lysates from M2. Molecular weights (Mr) are indicated in kDa.