Figure 9.

High N1-ICD levels in human breast cancers coexist with Fbxw7 thanks to high Pin1 expression.

A  Immunohistochemical analysis of the indicated proteins in a panel of 38 triple negative breast cancer samples. Upper panel: heatmap representing the protein levels of Pin1, Fbxw7α and N1-ICD in a cohort of 38 breast cancer patients. The colors represent high (red) or low (blue) protein levels according to protein expression scores (see supplementary Methods). Lower panel: Contingency table showing percentage of each category calculated on the precedent category of patients; chi-square test was performed for independence between the variables and the P-value = 10^–5.

B  NDT expression analysis in a Meta-dataset of 3254 breast cancer patients. Upper panel: heatmap representing the contingency table frequencies of samples classified as having high or low levels of FBXW7, of PIN1 and of the NDT gene signature. Number of samples in each category is indicated on the left. The association among high levels of NDT gene signature, PIN1, and FBXW7 resulted statistically significant (P < 0.001; chi-square test). Lower panel: Contingency table showing percentage of each category calculated on the precedent category of patients.

C  Expression correlation between NDT and PIN1 and FBXW7 mRNA levels. Average expression of NDT gene signature in breast cancer samples stratified according to high or low expression of PIN1 and FBXW7 mRNA. Data are shown as mean ± standard error of the mean (s.e.m.).

D  Survival analysis of Grade 3 high NDT expressing patients in function of PIN1 expression. Kaplan–Meier survival curve is indicated for high NDT signature, grade 3 breast cancer patients of the metadataset in function of high or low PIN1 mRNA levels. P-value and the number of subjects at risk at each time point is indicated below.