Figure 1.
A2MG-E microparticles enhance survival in murine sepsis and elevate exudate pro-resolving lipid mediator levels.
A A2MG containing microparticles (A2MG MP) were further enriched with A2MG (A2MG-E MP) and incorporation assessed by flow cytometry and ELISA.
B Kaplan–Meier survival analysis of mice subjected to CLP at time 0 (see Materials and Methods for details) and 1 h later treated with PBS (100 μl i.v.), soluble A2MG (sA2MG; 0.05 μg/mouse i.v.) or A2MG-E MP (1 × 105/mouse i.v.). Results are mean ± s.e.m., n = 12 mice per group (#P < 0.01, ##P < 0.001 versus PBS treated mice, one tailed log rank test).
C–E CLP mice were treated as in B and sacrificed at 12 h. Lipid mediators in peritoneal exudates were assessed using lipid mediator metabololipidomics following solid phase extraction (see Materials and Methods for details).
C Representative multiple reaction monitoring chromatograms of the identified lipid mediators in the peritoneal exudates.
D Accompanying MS-MS spectra employed for identification.
E Quantification of the identified lipid mediators was achieved by multiple reaction monitoring using parent ion and diagnostic ion in the MS-MS (daughter ion). Results for E are mean ± s.e.m., n = 4–5 mice per group (*P < 0.05 versus PBS treated mice by one way ANOVA).