FIG. 7.

In vivo bone-forming potential. Photomicrographs of decalcified paraffin-embedded sections under bright field illumination. (A) Comparing controls of scaffold (s) without cells versus (B) scaffold with cGMP-hBM-MSC previously maintained in culture medium. Regions adjacent to the scaffold containing newly formed bone osteoid matrix (b). Osteocytes were observed within lacunae (arrow). (C–E) 10×and corresponding 40×area enlargements. Photomicrographs of tissue sections from scaffolds implanted with cGMP-BM-MSC kept under shipment buffers with (C) 4% HSA#1; (D) 4% HSA#2; or (E) NS. Osteoid bone (b) formed on the scaffold (s) surrounded marrow-like regions (m). Representative sections (F–H) showed donor heterogeneity with regard to the relative amount of bone formed with (I) the corresponding histogram showing statistically significant differences (*^, **p<0.05). Scale bar=100 μm. Immunostaining with NOVA red chromogen-identified cells lining the newly formed bone matrix osteoid positive for (J) collagen type (I) A2 and (K) osteocalcin in contrast to the unstained (L) control. Color images available online at www.liebertpub.com/tec