Table 3.

Molecular interactions between EsAP3-2, EsPI and AP1/SEP/AGL6 superclade proteins tested by the yeast three-hybrid method

Ternary complex	-Leu Trp His + 25 mm 3AT	Ternary complex	-Leu Trp His + 25 mm 3AT
AD-EsFUL + pTFT1-EsAP3-2 + BD-EsPI	+	AD-EsAGL2-1 + pTFT1-AP3-2 BD-EsPI	+
AD-EsAGL6-like + pTFT1-EsAP3-2 + BD-EsPI	+	AD + pTFT1-AP3-2 + BD-EsPI	–
AD-EsFUL + pTFT1-EsAP3-2 + BD	–	AD-EsAGL2-1 + pTFT1-AP3-2 + BD	–

The class B genes EsAP3-2 and EsPI were inserted into pTFT-1 and pGBKT7 vectors, respectively, whereas the EsFUL-like, EsAGL2-1 and EsAGL6-like genes were inserted into the pGADT7 vector. +, strong interaction; –/ + , weak interaction; –, no interaction; N, no test.