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. 2012 Nov 14;32(46):16181–16192. doi: 10.1523/JNEUROSCI.0228-12.2012

Figure 1.

Figure 1.

dBACE mRNA and protein levels. A, Western blot using an anti-dBACE serum shows expression of the ∼38 kDa dBACE protein in head extracts from wild-type (WT) flies (lanes 1 and 3). Neither dBACE2045 (lane 2) nor dBACE2525 (lane 4) show significant reductions in the levels of dBACE, whereas dBACE is reduced in heterozygous dBACE5243 flies (lane 5) when compared with WT. B, No mRNA-derived dBACE PCR product is detectable in flies expressing the UAS-dBACERNAi construct via actin-GAL4 (lane 1). In contrast, it is present in flies carrying only the UAS-dBACERNAi or the actin-GAL4 construct (lanes 2 and 3). A control reaction using primers against the swiss-cheese transcript is shown below. C, Flies expressing UAS-dBACE with GMR-GAL4 show a strong expression of dBACE (lane 1, arrow) compared with WT (lane 2). GMR-GAL4; UAS-dBACERNAi knockdown flies show lower levels of dBACE (lane 3). Bottom, Loading controls for the Western blots using anti-tubulin.