Figure 6.

Loss of dBACE causes apoptotic cell death of glia. A, B, Vibratome sections stained with anti-cleaved caspase-3. A, Whereas a 3- to 5-d-old wild-type control does not show any anti-caspase staining (the weak green autofluorescence is caused by the eye pigment), several immunopositive cells are detectable in the lamina cortex of an age-matched GMR-GAL4; UAS-dBACE^RNAi fly (B, C), including cells in the subretinal region (arrows). The arrowhead points to a more proximally localized cell. D–F, Three-day-old GMR-GAL4; UAS-dBACE^RNAi fly double stained with anti-cleaved caspase-3 and anti-REPO. As seen in D (arrows) all the caspase-positive cells (red) are also labeled by anti-REPO (green) and most of them are found in the layer adjacent to the retina (arrows). Cells only stained by anti-REPO are mostly localized in more proximal layers (arrowheads). A, B, and C are 2 μm confocal sections and D–F is a 1 μm section. re, retina; la, lamina. Scale bars: (in A) 10 μm; (in C) 5 μm; (in D) 4 μm.