Figure 7.

dBACE is expressed in neurons. A, B, Vibratome sections stained with anti-dBACE reveal expression in the brain cortex (arrow in A; stack of 25 0.5 μm confocal sections) and in the neuropils of the optic system (A, arrowheads) and central brain (B, shown is a stack of 11 0.5 μm confocal sections). C–F, Single 0.5 μm confocal sections. Shown are dBACE staining (left), staining against the indicated marker, and the overlay of both stainings (right). C, A double staining with the neuronal marker 22C10 (red) reveals significant colocalization with dBACE (green), including staining in axons (arrowheads). Shown is the region indicated by the red box in A. D, E, dBACE (green) also colocalizes with APPL (red) in the retina (E) and in the brain, including staining in axons (D, arrows) and cell bodies (D, arrowheads). The region shown in D is indicated by the green box in A. F, A glial marker (mCD8-GFP expressed by the glial repo-GAL4, in red) shows limited colocalization with dBACE (green) in a few areas (arrows). The region shown is indicated by the blue box in B. la, lamina; me, medulla; lo, lobula; lp, lobula plate; cc, central complex. Scale bar, 20 μm.