. 2012 Nov 14;32(46):16181–16192. doi: 10.1523/JNEUROSCI.0228-12.2012

Table 1.

Summary of the degeneration measurements

Genotype/age	% of lamina filled by vacuoles	SEM	Analyzed brain hemispheres	% with vacuoles	Vacuoles in lamina
Canton S	0.485	0.233	28	21	Cortex
GMR–GAL4 28 d	0.716	0.2476	14	79	Cortex
dBACE^RNAi 28 d	0.427	0.2288	7	43	Cortex
GMR-GAL4; dBACE^RNAi 3 d	0.491	0.1118	32	50	Cortex
GMR-GAL4; dBACE ^RNAi 14 d	1.574	0.1996	12	100	Cortex
GMR-GAL4; dBACE^RNAi 28 d	3.614	0.2377	56	100	Cortex
GMR-GAL4; UAS-dBACE 28 d	2.748	0.3852	18	100	Cortex neuropil
GMR-GAL4; dBACE^RNAi ; UAS-dBACE 28 d	0.555	0.0919	30	70	Cortex neuropil
Appl-GAL4; dBACE^RNAi 28 d	1.547	0.1371	29	100	Cortex
dBACE²⁰⁴⁵/Df 28 d	3.502	0.5951	21	90	Cortex neuropil
dBACE²⁰⁴⁵/dBACE⁵²⁴³ 28 d	3.492	0.2959	21	100	Cortex neuropil
dBACE²⁵²⁵/dBACE⁵²⁴³ 28 d	1.494	0.1277	7	100	Cortex neuropil
Elav-GAL4; UAS-dBACE dBACE²⁰⁴⁵/Df 28 d	0.915	0.3112	8	62	Cortex neuropil
dBACE5.4-GAL4; UAS-dBACE dBACE²⁰⁴⁵/Df 28 d	0.501	0.1610	21	47	Cortex neuropil
GMR-GAL4; dBACE ^RNAi; UAS-GFP 28 d	3.292	0.2086	14	100	Cortex
GMR-GAL4;dBACE^RNAi; UAS-APPL 28 d	3.362	0.2193	26	100	Cortex neuropil
GMR-GAL4; dBACE^RNAi; UAS-APPL^SD 28 d	8.336	0.4886	14	100	Cortex neuropil
Appl^d; GMR-GAL4; dBACE^RNAi; 28 d	1.750	0.1818	18	100	Cortex
GMR-GAL4; UAS-GFP 28 d	0.799	0.2374	12	75	Cortex
GMR-GAL4; UAS-APPL 28 d	1.147	0.1907	19	94	Cortex
GMR-GAL4; UAS-APPL^SD 28 d	2.348	0.1585	15	100	Cortex neuropil
Appl^d; GMR-GAL4; 28 d	1.826	0.4899	14	100	Cortex

Vacuoles in central brain	Area of vacuoles in μm²	SEM	Analyzed brain hemispheres	% with vacuoles	p value
Appl-GAL4 28 d	4.7	0.887	20	90
Appl-GAL4; dBACE ^RNAi 28 d	34.6	3.882	12	100	<0.001
dBACE²⁰⁴⁵/dBACE⁵²⁴³ 28 d	18.0	2.911	13	100	<0.001

Note that even wild-type Canton S and the GMR-GAL4 and UAS-dBACE^RNAi flies show some vacuolization in the lamina cortex, possibly due to the presence of large trachea in this area, which appear as vacuoles in the paraffin sections.