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. Author manuscript; available in PMC: 2015 Mar 1.

Published in final edited form as: Pain. 2013 Nov 21;155(3):476–484. doi: 10.1016/j.pain.2013.11.007

Artemin blocks neurochemical signs in the DRG of nerve crush-induced neuropathy 6 weeks following injury. A. Nerve crush resulted in increased NPY labeling and decreased CGRP as well as IB4 labeling within the ipsilateral L₅ DRG. Artemin attenuated the nerve-crush induced increase in NPY and the decreased CGRP and IB4 labeling. B. The graph shows quantification of NPY, CGRP and IB4 labeled neuronal profiles in the ipsilateral L₅ DRG. The counts confirm that artemin ameliorated the nerve-crush induced increase in NPY and the decreased CGRP and IB4 labeling. Asterisks indicate significant differences (P < 0.05) compared to vehicle treatment after nerve crush. C. Nerve crush induced ATF3 labeled neuronal profiles were markedly reduced with artemin treatment. A similar decrease in nerve-crush induced caspase-3 labeled neuronal profiles was observed in artemin treated rats. Scale bar indicates 50 µm for all images. D. The graph shows quantification of ATF3 and caspase-3 labeled neuronal nuclei from counted sections confirming that artemin decreased the percentage of ATF3 and caspase-3 labeled neurons following nerve crush. Asterisks indicates significant differences (P < 0.05) compared to vehicle treatment after nerve crush.

Artemin blocks neurochemical signs in the DRG of nerve crush-induced neuropathy 6 weeks following injury. A. Nerve crush resulted in increased NPY labeling and decreased CGRP as well as IB4 labeling within the ipsilateral L₅ DRG. Artemin attenuated the nerve-crush induced increase in NPY and the decreased CGRP and IB4 labeling. B. The graph shows quantification of NPY, CGRP and IB4 labeled neuronal profiles in the ipsilateral L₅ DRG. The counts confirm that artemin ameliorated the nerve-crush induced increase in NPY and the decreased CGRP and IB4 labeling. Asterisks indicate significant differences (P < 0.05) compared to vehicle treatment after nerve crush. C. Nerve crush induced ATF3 labeled neuronal profiles were markedly reduced with artemin treatment. A similar decrease in nerve-crush induced caspase-3 labeled neuronal profiles was observed in artemin treated rats. Scale bar indicates 50 µm for all images. D. The graph shows quantification of ATF3 and caspase-3 labeled neuronal nuclei from counted sections confirming that artemin decreased the percentage of ATF3 and caspase-3 labeled neurons following nerve crush. Asterisks indicates significant differences (P < 0.05) compared to vehicle treatment after nerve crush.