Table 1.
Primary Antibody Characterization
| Protein | Immunogen | Technique/Dilution | Source | Host | Reference |
|---|---|---|---|---|---|
| Dynamin-1 | Synthetic peptide corresponding to AA residues 633–647 from rat/mouse/human dynamin-1 (EKASETEENGSDSF) | IHC 1:200, WB 1:1,000 and IP 10μg per 500μl retinal lysate | Thermo Scientific, (Rockford, Illinois; PA1-660) | rabbit polyclonal | (Kinuta et al., 2002; Ferguson et al., 2007) |
| Dynamin-2 | Synthetic peptide corresponding to AA residues 760–779 from rat/mouse/human dynamin-2 (SPTPQRRPVSSIHPPGRPPA) | IHC 1:200 and WB 1:1,000 | Thermo Scientific, (Rockford, Illinois; PA1-661) | rabbit polyclonal | (Sun et al., 2002; Lundmark & Carlsson, 2003; Kelly et al., 2005) |
| Dynamin-3 | Synthetic C-terminal peptide, specific to dynamin-3 (RLTLSAPLPRPASSRGPAPAIPSPGPHS) | IHC 1:200 and WB 1:1,000 | Dr. Mark A. McNiven, (Mayo Clinic, Minneapolis, MN) | rabbit polyclonal | (Cao et al., 1998; Gray et al., 2003; Vaid et al., 2007) |
| Pan-Dynamin | Synthetic peptide corresponding to AA residues 2–17 of dynamin 1,2 & 3 in rat/mouse (GNRGMEDLIPLVNRLQ) | IHC 1:100 | Synaptic Systems, (Gottingen, Germany; 115 002) | rabbit polyclonal | (Holroyd et al., 2002; Ferguson et al., 2007; Rozas et al., 2011) |
| Tulp1 | Synthetic peptide corresponding to N-terminal AA residues 1–201 of mouse Tulp1 | WB 1:1,000 | Dr. Stephanie A. Hagstrom, (Mass. Eye and Ear Infirmary, Boston, MA) | rabbit polyclonal | (Hagstrom et al., 2001; Xi et al., 2007; Grossman et al., 2009) |
| Peripherin | AA residues of the C-terminus of mouse Peripherin (CVEAEGADAGPAPEAG) | IHC 1:500 and WB 1:500 | Dr. Andrew F.X. Goldberg, (Oakland Univ., Rochester, MI) | rabbit polyclonal | (Goldberg et al., 2007) |
| Clathrin | Full length native bovine protein (purified), which recognizes AAs 207–264 of bovine clathrin heavy chains | WB 1:500 | Novus Biologicals (Littleton, CO;NB120-11331 | mouse monoclonal | (http://www.novusbio.com/Clathrin-heavy-chain-Antibody-TD1_NB120-11331.html) |
| Lima1 | AA residues 700–759 corresponding to human Lima1 | WB 1:500 | Bethyl Laboratories (Montgomery, TX; 0-103A) | rabbit polyclonal | (Han et al., 2007) |
| Myelin Basic Protein | Myelin basic protein isolated from human brain | WB 1:200 | Dako (Carpinteria, CA; A0623) | rabbit polyclonal | (Werner et al., 2007) |
| Myosin 10 | Synthetic peptide corresponding to the C-terminus of human Myosin 10 (Myosin IIB) | WB 1:500 | Cell Signaling Technology (Boston, MA;3404S) | rabbit polyclonal | (Daley et al., 2009) |
| Rhodopsin | 15 AA residues of N-terminal of bovine rhodopsin (MNGTEGPNFYVPFSN) | WB 1:1,000 | Dr. Paul Hargrave (University of Florida, Gainesville, FL;R2-15N) | mouse monoclonal | (Adamus et al., 1991) |
| Spectrin | cell membranes purified by hypotonic lysis and mechanical enucleation | WB 1:400 | EMD Millipore (Billerica, MA; MAB1622-Clone AA6) | mouse monoclonal | (Nakajima et al., 2011) |
| Rootletin | AA residues 1819–2006 of mouse rootletin | WB 1:1000 | Dr. Tiansen Li, (National Eye Institute, Bethesda, MD) | rabbit polyclonal | (Yang et al., 2002) |