Table 1.
Primers for RT-PCR
| Gene | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|
| G3PDH |
ACCACAGTCCATGCCATCAC |
TCCACCACCCTGTTGCTGTA |
452 |
| PAR-1 |
GGATATTTGACCAGCTCCTGG |
AGATGGCCAGACAAGTGAAGG |
400 |
| PAR-2 |
CTGCATCTGTCCTCACTGGAA |
ATTGCCAGGGAGATGCCAATG |
400 |
| Claudin-1 |
GCTGCTGGGTTTCATCCTG |
CACATAGTCTTTCCCACTAGAAG |
619 |
| Claudin-4 |
AGCCTTCCAGGTCCTCAACT |
AGCAGCGAGTAGAAG |
249 |
| Claudin-7 |
AGGCATAATTTTCATCGTGG |
GAGTTGGACTTAGGGTAAGAGCG |
252 |
| Occludin |
TCAGGGAATATCCACCTATCACTTCAG |
CATCAGCAGCAGCCATGTACTCTTCAC |
189 |
| Tricellulin | AGGCAGCTCGGAGACATAGA | TCACAGGGTATTTTGCCACA | 200 |