Table 2.
Relative mRNA concentrations of ER stress-induced genes in the liver of Holstein cows at 3 wk antepartum and 1, 5 and 14 wk postpartum 1
Gene 2 | 3 wk antepartum (n = 13) | 1 wk postpartum (n = 13) | 5 wk postpartum (n = 13) | 14 wk postpartum (n = 13) |
---|---|---|---|---|
ATF4 |
1.00 ± 0.25a |
3.14 ± 0.25c |
2.08 ± 0.26b |
1.65 ± 0.24b |
BAK1 |
1.00 ± 0.29a |
2.10 ± 0.30b |
1.56 ± 0.29ab |
1.59 ± 0.28ab |
BAX |
1.00 ± 0.36a |
3.02 ± 0.39b |
1.98 ± 0.35ab |
1.52 ± 0.35a |
CASP3 |
1.00 ± 0.31a |
2.54 ± 0.27b |
2.07 ± 0.26b |
1.71 ± 0.26ab |
CASP8 |
1.00 ± 0.25a |
2.48 ± 0.24b |
1.88 ± 0.23ab |
1.73 ± 0.23ab |
CASP9 |
1.00 ± 0.37a |
2.45 ± 0.35b |
1.89 ± 0.34ab |
1.84 ± 0.36ab |
DDIT3 |
1.00 ± 0.17a |
1.86 ± 0.17b |
1.58 ± 0.17ab |
1.41 ± 0.17ab |
DNAJC3 |
1.00 ± 0.15a |
1.89 ± 0.17b |
1.49 ± 0.17ab |
1.43 ± 0.15ab |
EDEM1 |
1.00 ± 0.21a |
1.70 ± 0.21b |
1.69 ± 0.20b |
1.25 ± 0.22ab |
HERPUD1 |
1.00 ± 0.45a |
3.96 ± 0.37b |
2.27 ± 0.43a |
1.66 ± 0.38a |
HSPA5 |
1.00 ± 0.29a |
2.74 ± 0.27b |
1.64 ± 0.26a |
1.41 ± 0.24a |
PDIA4 |
1.00 ± 0.25a |
1.92 ± 0.28b |
1.34 ± 0.24ab |
1.23 ± 0.25ab |
WARS |
1.00 ± 0.35a |
2.03 ± 0.31ab |
2.31 ± 0.29b |
1.93 ± 0.30ab |
XBP1 (unspliced) | 1.00 ± 0.25a | 3.14 ± 0.25c | 2.08 ± 0.26b | 1.65 ± 0.24b |
1mRNA concentrations of genes are expressed relative to the mRNA abundance at 3 wk antepartum.
2Abbreviations of gene names: see Table 1.
Values are means ± SEM.
a,b,cMeans without common superscripts differ significantly (P < 0.05).