Table 4.
Characteristics of gene specific primers used for qPCR
| Gene 1 | Forward primer (from 5′ to 3′) Reverse primer (from 5′ to 3′) | PCR product size (bp) | NCBI GenBank |
|---|---|---|---|
| Reference genes |
|
|
|
|
ATP5B |
GGACTCAGCCCTTCAGCGCC GCCTGGTCTCCCTGCCTTGC |
229 |
NM_175796.2 |
|
PPIA |
GGCAAATGCTGGCCCCAACACA AGTACCACGTGCTTGCCATCCA |
87 |
NM_178320.2 |
|
RPL12 |
CACCAGCCGCCTCCACCATG CGACTTCCCCACCGGTGCAC |
84 |
NM_205797.1 |
| Target genes |
|
|
|
|
ATF4 |
TGGTCTCAGACAACAGCAAG AGCTCATCTGGCATGGTTTC |
130 |
NM_001034342.2 |
|
ATM |
GTGTTGAGGCACTTTGTGATGC GTTTGATAATGGGCTGGTCTGC |
111 |
NM_001205935.1 |
|
BAK1 |
TACTTCACCAAGATCGCGTC ACGATGGCTACGCTCTTGAT |
254 |
NM_001077918.1 |
|
BAX |
TCTGACGGCAACTTCAACTG ATGGTCACTGTCTGCCATGT |
224 |
NM_173894.1 |
|
BRCA1 |
TGCCGAGACAAGATCAAGAGG TAATTTCAGTGCAGAGGCTGAGG |
149 |
NM_178573.1 |
|
CASP3 |
CCGAGGCACAGAACTGGACTG TCGCCAGGAAAAGTAACCAGGTG |
133 |
NM_001077840.1 |
|
CASP8 |
TACCAGCGAGGAGGAGATGAAG CATCCAGCTTACATTTGGCAATC |
164 |
NM_001045970.2 |
|
CASP9 |
AAACAGGATGACCCATCAAAGC ATTCAGGACATAGGCCAGATCG |
203 |
NM_001205504.1 |
|
DDIT3 |
AGTCACTGCCTTTCTCCTTC TCTTCCTCCTTGTTTCCAGG |
133 |
NM_001078163.1 |
|
DNAJC3 |
GTACGAAGGTGCTGAATGTG ATCAGGGTCACCATCTACTG |
133 |
NM_174756.3 |
|
EDEM1 |
CCCCTACCCTCGGGTGAATCT GTGGAATCCCCCAGCAGTCG |
126 |
NM_001103092.2 |
|
HERPUD1 |
CCGTGTTTCTCAGTATCCTC TCTTGATTCACAGCCTCCTG |
169 |
NM_001102265.2 |
|
HSBP1 |
CGCGAACAAACGGAAGTATAGG CAGGTCATCAATGCGACTGC |
207 |
NM_001113316.2 |
|
HSPA5 |
CAAGTTGATGTTGGAGGTGG AAGCCTCAGCAGTTTCCTTC |
94 |
NM_001075148.1 |
|
HSPA8 |
AACGTGCTGATCTTTGATTTAGGG TTCTCCACCCAAGTGAGTATCTCC |
114 |
NM_174345.4 |
|
MSH2 |
AACAGAAAGCCCTGGAGTTGG TTATTCTTTGCGATGACCTCAGC |
226 |
NM_001034584.1 |
|
PDIA4 |
AGGTTTGACGTGAGTGGCTA CATCGAAGTTGTCCTTGGTC |
175 |
NM_001045879.2 |
|
RPS9 |
GTGAGGTCTGGAGGGTCAAA GGGCATTACCTTCGAACAGA |
108 |
NM_001101152.2 |
|
WARS |
AAGCAGACGAGGACTTTGTG TTCGGTTTACCAGCTCCTTG |
123 |
NM_174218.1 |
|
XBP1 unspliced |
GTTGAGACAGCGGTTGGGAATG CGTAGTCTGAGTGCTGCGGAC |
114 |
NM_001034727.3 |
|
XBP1 spliced & unspliced |
TGACTGAAGAGGAAGCAGAG CAATGCCATCAGAGTCCATG |
129/155 |
NM_001271737.1 |
| XRCC5 | GTTTCAGTGTCTGCTTCACAGAGC TTCTTGATGACTTCCGTCAGAGG | 165 | NM_001102141.1 |