Figure 4.

Temperature-dependent DNA affinity of MADS-domain complexes. Relative binding affinity of three MADS-domain complexes to a probe representing the AGAMOUS intron relative to the affinity to a probe representing the same region but with the A-tract element inside of the CArG-box mutated (see ‘Materials and Methods’ section). The relative affinity was studied at different temperatures by QuMFRA experiments. Error bars indicates standard error calculated out of six replicates. Supplementary Figure S8 shows the images of gels of two replicates from which these affinities were calculated.