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. 2013 Nov 27;42(4):2224–2234. doi: 10.1093/nar/gkt1230

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Gcn4 site activates in AAS and represses in glucose, but Gcn4 TF is an activator in both conditions. Strains bearing synthetic promoters with avi-tagged Gcn4 were grown as described in Methods in glucose and AAS media. Expression was measured via flow cytometry. Occupancy was measured by ChIP. (A) A boxplot of expression of promoters in AAS (left) and glucose (right) grouped by the number of Gcn4 sites present in the promoter shows that Gcn4 sites repress weakly in glucose but activate strongly in AAS. (B) A plot of expression versus total Gcn4 occupancy in AAS (left) and glucose (right) shows that Gcn4 occupancy correlates positively with expression in both AAS and glucose. This suggests a repressive factor is binding the Gcn4 site in glucose in competition with Gcn4. There are fewer points in AAS than in glucose due to the strong activating effect of multiple Gcn4 sites in AAS.