Table 1.
Summary of usable promoters for expression analysis
| Tagged TF | Total, glucose | Unique, glucose | Total, AAS | Unique, AAS |
|---|---|---|---|---|
| Cbf1 | 529 | 218 | 374 | 125 |
| Gcn4 | 614 | 213 | 396 | 114 |
| Met31a | 643 | 271 | 475 | 170 |
| Nrg1a | 634 | 271 | 393 | 139 |
Cbf1, Gcn4, Met31 and Nrg1 were tagged with the myc-C-avi tag in a strain harboring the bacterial biotin ligase BirA.
Synthetic promoters containing sites for all four factors were constructed in each strain. Nine hundred sixty colonies were picked for each library, purified, sequenced and then grown in glucose and AAS. The library members were cross-linked, and then run on a Beckman Coulter Cell Lab Quanta SC flow cytometer to measure the fluorescence of the reporter gene in each strain. The numbers shown are the number of strains for which sequence information was determined and for which a reliable fluorescence value was obtained. aOmitted from expression analysis due to lack of ChIP signal from occupancy analysis.