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. 2013 Nov 22;42(4):e29. doi: 10.1093/nar/gkt1179

Figure 5.

Figure 5.

Sensitivity comparison of the biotinylation and radiolabeling methods. (A) Excised oligonucleotides containing UV damage were recovered from A375 cells at various time points after exposure to 10 J/m2 of UV-C. The purified oligonucleotides were then labeled with biotin-11-dUTP or cordycepin 5′-triphosphate at the 3′-end using terminal deoxynucleotidyl transferase (TdT). Biotin-labeled DNAs were separated on a 12% TBE-urea gel, transferred to a nylon membrane, and detected with HRP-conjugated streptavidin-HRP using a chemiluminescence reagent. Radiolabeled DNAs were separated on a 12% TBE-urea gel and detected by exposing the gel to a phosphorimager screen. (B) Quantitative analysis of the excised repair products. The results from three independent experiments were quantified and are plotted (means ± standard deviation) relative to the maximum.