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. 2013 Dec 5;42(4):2380–2390. doi: 10.1093/nar/gkt1263

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effect of RECQ5 deficiency on RAD51 occupancy at chromatin flanking the I-SceI-induced DSB in U2OS/DR-GFP cells. (A) A schematic diagram of a part of the DR-GFP reporter cassette showing the locations of the regions amplified in ChIP-qPCR assays (P1 and P2). The GFP open reading frame with a single I-SceI recognition site is shown as gray box. The numbers correspond to base pairs. (B) Plot of ChIP-qPCR data. Mock-depleted (siLuc) and RECQ5-depleted (siRECQ5#1) cells were transfected with either I-SceI expression vector (+I-SceI) or empty vector, respectively. Chromatin for ChIP analysis was prepared 2 days after I-SceI transfection. Fold enrichment was calculated as a ratio of RAD51 antibody signal versus control IgG. ChIP, chromatin immunoprecipitation; and qPCR, quantitative real-time PCR.