Table 1.
Transposition frequencies of individual mutants
| Mutation | W | V | P | H | E | L | |
|---|---|---|---|---|---|---|---|
| Mutant/W.T. | Mutant/W.T. | Mutant/W.T. | Mutant/W.T. | Mutant/W.T. | Mutant/W.T. | ||
| Small | G | 1 | 0.03 | nd | 0.08 | 0.4 | 20 |
| A | 8 | 3 | 0.5 | nd | 3 | 5 | |
| Nucleophilic | S | 20 | 7 | nd | 0.09 | 6 | 50 |
| T | 8 | 7 | nd | 0.3 | 8 | 20 | |
| C | 8 | 2 | 0.4 | nd | 1 | 0.7 | |
| Hydrophobic | V | 10 | 1 | 0.6 | nd | 10 | 6 |
| L | 6 | 0.1 | 0.4 | nd | 4 | 1 | |
| I | 4 | 3 | nd | nd | 2 | 3 | |
| M | 10 | nd | nd | nd | nd | 0.8 | |
| P | 4 | 0.02 | 1 | nd | 0.2 | 0.06 | |
| Aromatic | F | 8 | 0.2 | nd | 0.4 | 2 | 0.09 |
| Y | nd | 0.06 | nd | 0.5 | 5 | nd | |
| W | 1 | nd | nd | 1 | 2 | nd | |
| Acidic | D | 0.3 | nd | nd | nd | 0.08 | 20 |
| E | 2 | 3 | nd | nd | 1 | 7 | |
| Amide | N | nd | 0.8 | nd | nd | 0.6 | 60 |
| Q | 8 | 2 | 0.01 | nd | 6 | 3 | |
| Basic | H | 10 | 0.3 | nd | 1 | 2 | 0.1 |
| K | 10 | 0.4 | 0.04 | 0.2 | 7 | nd | |
| R | 30 | 0.02 | nd | nd | 30 | 0.9 | |
Transposition frequencies were measured by the bacterial mating-out assay and are the average of three independent experiments. The transposition frequency is obtained by dividing the total number of transconjugants containing the transposon by the total number of transconjugants. Mutant/wild-type transposase (W.T.) figures are rounded to one significant figure. nd, not determined.