Figure 9.

MepR binding to the mepA operator. In all experiments, MepR was injected into dsDNA. The DNA concentration was 10 μM, the concentration of MepR in the first experiment was 272 μM, in the second and fourth, 200 μM, and in the third, 370 μM. A stoichiometry of 4 corresponds to 2 dimers of MepR binding to a single dsDNA molecule; a stoichiometry of 2 corresponds to 1 dimer of MepR binding to a single dsDNA molecule. In MepR binding to the 46 bp mepA operator in which the 2nd site is scrambled, only the first transition was fit. The sequences of the oligodeoxynucleotides and fitted thermodynamic parameters are provided in Supplementary Table S1.