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Effect of MLL1 depletion on the recruitment of ERαand FOXA1 to TFF1 EREs. (A-C) ChIP assays were performed as detailed in Figure 2 after transfection with siNS, siMLL1 or siFOXA1. The MCF-7 cells were treated with E2 (100 nM) or ethanol for the indicated times (A, C) or 60 min (B). The amount of the indicated region of the TFF1 gene precipitated by ERα, MLL1 or FOXA1 antibodies was determined by qPCR.
